Transcriptome-Wide Mapping of N 1-Methyladenosine Methylome

Methods Mol Biol. 2017:1562:245-255. doi: 10.1007/978-1-4939-6807-7_16.

Abstract

N 1-Methyladenosine (m1A) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of m1A in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of m1A will be important for further functional investigations. Here, we describe a technique called "m1A-ID-Seq", which is based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, to map m1A in the transcriptome. Utilizing this technique, highly confident m1A peaks can be obtained on a transcriptome-wide level.

Keywords: AlkB; Demethylase; Epitranscriptome; N 1-Methyladenosine (m1A); RNA epigenetics; Transcriptome-wide profiling; m1A-ID-Seq.

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / chemistry
  • Adenosine / genetics
  • Adenosine / metabolism
  • AlkB Enzymes / metabolism
  • Epigenesis, Genetic*
  • Epigenomics / methods*
  • Gene Library
  • Immunoprecipitation / methods
  • Methylation
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Transcriptome*

Substances

  • RNA, Messenger
  • 1-methyladenosine
  • AlkB Enzymes
  • Adenosine