Reverse Phase Protein Arrays and Drug Discovery

Methods Mol Biol. 2017:1647:153-169. doi: 10.1007/978-1-4939-7201-2_10.

Abstract

Reverse Phase Protein Arrays (RPPA) represent a sensitive antibody-based proteomic approach, which enables simultaneous quantification of the abundance of multiple proteins and posttranslational modifications across multiple samples. Here, we provide protocols for RPPA performed on two distinct protein-binding substrates associated with two most commonly used RPPA platform technologies. We compare and contrast the respective advantages and limitations of each platform within the context of drug discovery applications.

Keywords: Antibody; Array; Multiplex; Protein; Proteomics.

MeSH terms

  • Biomarkers, Tumor / analysis
  • Collodion / chemistry
  • Drug Discovery / methods*
  • Humans
  • Protein Array Analysis / methods*
  • Protein Binding
  • Protein Processing, Post-Translational
  • Proteins / analysis*
  • Proteins / chemistry
  • Small Molecule Libraries / analysis
  • Tumor Cells, Cultured

Substances

  • Biomarkers, Tumor
  • Proteins
  • Small Molecule Libraries
  • Collodion