Parent-of-origin effects play important roles in plant reproduction and are often mediated by epigenetic modifications at the histone or DNA level. However, the genetic basis underlying these modifications can be challenging to identify. Here, we describe an approach (Bulk-Seq) that can be used to map loci mediating parent-of-origin-dependent effects using whole-genome sequencing of pools of DNA.
Keywords: Arabidopsis thaliana; Bulk-segregant sequencing; Parent-of-origin effects; Quantitative trait mapping; Seed development; Segregation distortion.