Inactivation of phi X174 DNA by the ortho-quinone derivative or its reduction product of the antitumor agent VP 16-213

J M van Maanen; C de Ruiter; P R Kootstra; M V Lafleur; J de Vries; J Retèl; H M Pinedo

doi:10.1016/0277-5379(85)90018-5

Inactivation of phi X174 DNA by the ortho-quinone derivative or its reduction product of the antitumor agent VP 16-213

Eur J Cancer Clin Oncol. 1985 Oct;21(10):1215-8. doi: 10.1016/0277-5379(85)90018-5.

Authors

J M van Maanen, C de Ruiter, P R Kootstra, M V Lafleur, J de Vries, J Retèl, H M Pinedo

PMID: 2934259
DOI: 10.1016/0277-5379(85)90018-5

Abstract

Biologically active phi X174 DNA is inactivated by the ortho-quinone derivative of the antitumor agent VP 16-213, but not by VP 16-213 itself, VP 16-213 phenoxy radical or aqueous decomposition product(s) of the ortho-quinone. Reduction of the ortho-quinone by cytochrome P-450 reductase and NADPH results in deactivation of the ortho-quinone towards anti-phi X174 DNA activity. However, compared with the parent compound VP 16-213, reduction of the ortho-quinone results in substantial damage towards DNA.

MeSH terms

Bacteriophage phi X 174 / drug effects*
DNA, Single-Stranded
DNA, Viral*
Etoposide / analogs & derivatives*
Etoposide / pharmacology
NADP / metabolism
Podophyllotoxin / analogs & derivatives*

Substances

DNA, Single-Stranded
DNA, Viral
NADP
Etoposide
Podophyllotoxin