Abstract
We measured the inhibition of forskolin-stimulated adenylate cyclase activity in guinea pig hippocampal membranes by 5-HT, 5-carboxamidotryptamine (CAT) and 8-hydroxy-2-(di-n-propylamino) tetralin (PAT). Low concentrations of these agonists inhibited forskolin-stimulated adenylate cyclase activity in a concentration-dependent and saturable manner. The antagonist spiperone shifted the concentration-response curve to CAT to the right in a parallel manner. The EC50 values of CAT, PAT and 5-HT and the KB of spiperone suggest that this receptor may correspond to the 5-HT1A binding site.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Hydroxy-2-(di-n-propylamino)tetralin
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Adenylyl Cyclase Inhibitors*
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Adenylyl Cyclases / metabolism
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Animals
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Colforsin / antagonists & inhibitors
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Colforsin / pharmacology*
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Dose-Response Relationship, Drug
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Guinea Pigs
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Hippocampus / drug effects
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Hippocampus / enzymology
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In Vitro Techniques
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Male
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Receptors, Serotonin / drug effects
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Receptors, Serotonin / physiology*
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Serotonin / analogs & derivatives
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Serotonin / pharmacology
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Serotonin Antagonists / pharmacology
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Spiperone / pharmacology
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Tetrahydronaphthalenes / antagonists & inhibitors
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Tetrahydronaphthalenes / pharmacology
Substances
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Adenylyl Cyclase Inhibitors
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Receptors, Serotonin
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Serotonin Antagonists
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Tetrahydronaphthalenes
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Colforsin
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Serotonin
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Spiperone
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8-Hydroxy-2-(di-n-propylamino)tetralin
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5-carboxamidotryptamine
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Adenylyl Cyclases