+mRNA expression of LRRC55 protein (leucine-rich repeat-containing protein 55) in the adult mouse brain

Ying-Ying Zhang; Xue Han; Ye Liu; Jian Chen; Lei Hua; Qian Ma; Yang-Yu-Xin Huang; Qiong-Yao Tang; Zhe Zhang

doi:10.1371/journal.pone.0191749

+mRNA expression of LRRC55 protein (leucine-rich repeat-containing protein 55) in the adult mouse brain

PLoS One. 2018 Jan 25;13(1):e0191749. doi: 10.1371/journal.pone.0191749. eCollection 2018.

Authors

Ying-Ying Zhang¹, Xue Han¹, Ye Liu^{2

3}, Jian Chen¹, Lei Hua¹, Qian Ma¹, Yang-Yu-Xin Huang¹, Qiong-Yao Tang^{1

2

3}, Zhe Zhang^{1

2

3}

Affiliations

¹ School of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
² Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
³ Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.

Abstract

LRRC55 (leucine-rich repeat-containing protein 55) protein is an auxiliary γ subunit of BK (Big conductance potassium channel) channels, which leftward shifts GVs of BK channels around 50 mV in the absence of cytosolic Ca2+. LRRC55 protein is also the only γ subunit of BK channels that is expressed in mammalian nervous system. However, the expression pattern of LRRC55 gene in adult mammalian brain remains elusive. In this study, we investigated the distribution of LRRC55 mRNA in the adult mouse brain by using in situ hybridization. We found that LRRC55 mRNA is richly expressed in the adult mouse medial habenula nucleus (MHb), cerebellum and pons. However, the potential role of LRRC55 in MHb and cerebellum could be different based on the function of BK channels in these brain regions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
In Situ Hybridization
Membrane Proteins / genetics*
Mice
Mice, Inbred C57BL
RNA, Messenger / genetics*

Substances

Membrane Proteins
RNA, Messenger
leucine-rich repeat-containing protein 55, mouse

Grants and funding

This work was supported by The Important Project of Natural Science in Colleges and Universities in Jiangsu Province to Z.Z. (14KJA320002), Jiangsu specially appointed professorship to Z.Z. and Q.-Y.T., Natural Science Foundation of China (NSFC) grant to Z.Z. (81471314, 81671090), Natural Science Foundation of Jiangsu Province to Z.Z (SBK201502515). Xuzhou Science and Technology Program (16631635) to Z.Z. NSFC grant to Q.-Y.T. (C110307). Training programs of innovation and Entrepreneurship for undergraduates of Jiangsu Province in 2014 (201410313065X). We also appreciated the grant support from the Priority Academic Program Development of Jiangsu Higher Education Institutions and from the Jiangsu Provincial Special Program of Medical Science (BL2014029). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.