Study of Autophagy in Plant Senescence

Xuefei Cui; Jing Zheng; Jinxin Zheng; Qingqiu Gong

doi:10.1007/978-1-4939-7672-0_23

Study of Autophagy in Plant Senescence

Methods Mol Biol. 2018:1744:299-306. doi: 10.1007/978-1-4939-7672-0_23.

Authors

Xuefei Cui^{1

2}, Jing Zheng^{1

2}, Jinxin Zheng^{1

2}, Qingqiu Gong^{3

4}

Affiliations

¹ Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
² Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, China.
³ Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China. gongq@nankai.edu.cn.
⁴ Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, China. gongq@nankai.edu.cn.

PMID: 29392674
DOI: 10.1007/978-1-4939-7672-0_23

Abstract

As a major intracellular degradation pathway, autophagy contributes to nutrient recycling and is indispensable during plant senescence. Here we describe methods used for investigating the autophagic process during leaf senescence. These include transcript analysis of core machinery autophagy genes, immunoblotting of ATG8, and microscopic observation of autophagosome formation.

Keywords: ATG8; Autophagy; Confocal laser scanning microscopy; Immunoblots; Leaf senescence; Transcript analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging*
Autophagy*
Gene Expression Profiling
Gene Expression Regulation, Plant
Microscopy, Confocal
Plant Development / genetics
Plant Leaves / physiology
Plant Physiological Phenomena*
Transcriptome