P38 activation induces the dissociation of tristetraprolin from Argonaute 2 to increase ARE-mRNA stabilization

Mol Biol Cell. 2018 Apr 15;29(8):988-1002. doi: 10.1091/mbc.E17-02-0105. Epub 2018 Mar 30.

Abstract

ARE-mRNAs are actively degraded with tristetraprolin (TTP) in resting cells while they turn into stable messengers in activated cells. P38 plays a crucial role in stabilizing ARE-mRNA. Here we reveal that P38 activation represses the interaction between TTP and Ago2, thus restraining TTP from being targeted into processing bodies and stabilizing ARE-mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • AU Rich Elements*
  • Argonaute Proteins / genetics
  • Argonaute Proteins / metabolism*
  • Gene Expression Regulation
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Lipopolysaccharides / pharmacology
  • Phosphorylation
  • RNA Stability*
  • RNA, Messenger / genetics*
  • Transcription, Genetic / drug effects
  • Tristetraprolin / genetics
  • Tristetraprolin / metabolism*
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

  • 3' Untranslated Regions
  • AGO2 protein, human
  • Argonaute Proteins
  • Lipopolysaccharides
  • RNA, Messenger
  • Tristetraprolin
  • Tumor Necrosis Factor-alpha
  • p38 Mitogen-Activated Protein Kinases