Mouse Genotyping

Sandra Vuong; Paul Delgado-Olguin

doi:10.1007/978-1-4939-7714-7_1

Mouse Genotyping

Methods Mol Biol. 2018:1752:1-9. doi: 10.1007/978-1-4939-7714-7_1.

Authors

Sandra Vuong^{1

2}, Paul Delgado-Olguin^{3

4

5}

Affiliations

¹ Translational Medicine, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G0A4, Canada.
² Department of Molecular Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, Canada.
³ Translational Medicine, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G0A4, Canada. paul.delgadoolguin@sickkids.ca.
⁴ Department of Molecular Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, Canada. paul.delgadoolguin@sickkids.ca.
⁵ Heart & Stroke Richard Lewar Centres of Excellence in Cardiovascular Research, 6 Queen's Park Cres W, Toronto, ON, M5S3H2, Canada. paul.delgadoolguin@sickkids.ca.

PMID: 29564757
DOI: 10.1007/978-1-4939-7714-7_1

Abstract

Genotyping is an invaluable tool for identifying organisms carrying genetic variations including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are analyzed by agarose gel electrophoresis based on their size.

Keywords: DNA; Genotype; Polymerase chain reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA / genetics*
Electrophoresis, Agar Gel
Genotype*
Mice
Polymerase Chain Reaction

Substances

DNA

Grants and funding

PJT-149046/CIHR/Canada