Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype

Michael Peitz; Tamara Bechler; Catrin Cornelia Thiele; Monika Veltel; Melanie Bloschies; Klaus Fliessbach; Alfredo Ramirez; Oliver Brüstle

doi:10.1016/j.scr.2018.04.011

Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype

Stem Cell Res. 2018 May:29:250-253. doi: 10.1016/j.scr.2018.04.011. Epub 2018 Apr 23.

Authors

Michael Peitz¹, Tamara Bechler², Catrin Cornelia Thiele¹, Monika Veltel², Melanie Bloschies², Klaus Fliessbach³, Alfredo Ramirez⁴, Oliver Brüstle⁵

Affiliations

¹ Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany,; DZNE German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany.
² Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany.
³ DZNE German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany,; Department of Neurodegenerative Diseases and Geriatric Psychiatry, University Hospital Bonn, Germany.
⁴ Department of Neurodegenerative Diseases and Geriatric Psychiatry, University Hospital Bonn, Germany,; Department of Psychiatry and Psychotherapy, University of Cologne, Cologne, Germany,; Department of Psychiatry and Psychotherapy, University of Bonn, Bonn, Germany.
⁵ Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany,. Electronic address: brustle@uni-bonn.de.

PMID: 29753274
DOI: 10.1016/j.scr.2018.04.011

Abstract

Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged, 80 and over
Alzheimer Disease* / diagnosis
Alzheimer Disease* / genetics
Alzheimer Disease* / metabolism
Alzheimer Disease* / pathology
Apolipoprotein E4* / genetics
Apolipoprotein E4* / metabolism
Blood Cells* / metabolism
Blood Cells* / pathology
Cellular Reprogramming Techniques
Genotype*
Humans
Induced Pluripotent Stem Cells* / metabolism
Induced Pluripotent Stem Cells* / pathology
Kruppel-Like Factor 4
Male
Polymorphism, Single Nucleotide*
Transcription Factors / biosynthesis
Transcription Factors / genetics

Substances

Apolipoprotein E4
KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors