Objective: To review the in vivo imaging research progress of two-photon microscopy (TPM) in spinal cord.
Methods: The recent literature concerning in vivo two-photon imaging of axon, microglia, and calcium in transgenic mice spinal cord was extensively consulted and reviewed.
Results: In vivo two-photon imaging of spinal cord provide dynamic information about axonal degeneration and regeneration, microglial accumulation, and calcium influx after spinal cord injury.
Conclusions: TPM in vivo imaging study on spinal cord will provide theoretical foundation for pathophysiologic process of spinal cord injury.
目的: 综述双光子显微镜（two-photon microscopy，TPM）活体脊髓成像领域的研究进展。.
方法: 广泛查阅近年来TPM对转基因小鼠脊髓轴突、小胶质细胞、钙离子活性进行活体成像的相关研究并进行分析总结。.
结果: 应用TPM能在长时间内观察活体内脊髓损伤后轴突退变与再生、小胶质细胞聚集、钙离子的动态变化。.
结论: TPM对脊髓损伤后细胞水平的活体成像，为理解脊髓损伤的病理生理过程奠定了重要的理论基础。.
Keywords: In vivo imaging; Spinal cord injury; Transgenic mouse; Two-photon microscopy.