Abstract
An (η5-cyclopentadienyl)cobalt(i) complex was covalently incorporated into an engineered variant of the transmembrane protein ferric hydroxamate uptake protein component: A, FhuA ΔCVFtev, using a thiol-ene reaction. A CD spectrum shows the structural integrity of the biohybrid catalyst. MALDI-TOF of the segment containing the anchoring site for the cobalt complex Cys545 confirmed successful conjugation. This biohybrid catalyst catalyzed the cyclotrimerization of phenylacetylene to give a mixture of regioisomeric 1,2,4- and 1,3,5-triphenylbenzene in aqueous medium.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetylene / analogs & derivatives*
-
Acetylene / chemistry
-
Acetylene / metabolism
-
Bacterial Outer Membrane Proteins / chemistry*
-
Bacterial Outer Membrane Proteins / genetics
-
Bacterial Outer Membrane Proteins / metabolism
-
Catalysis
-
Cobalt / chemistry*
-
Coordination Complexes / chemistry*
-
Cyclization
-
Dimerization
-
Escherichia coli / chemistry*
-
Escherichia coli / genetics
-
Escherichia coli / metabolism
-
Escherichia coli Proteins / chemistry*
-
Escherichia coli Proteins / genetics
-
Escherichia coli Proteins / metabolism
-
Ferric Compounds / metabolism
-
Hydroxamic Acids / metabolism
-
Models, Molecular
-
Protein Engineering
Substances
-
Bacterial Outer Membrane Proteins
-
Coordination Complexes
-
Escherichia coli Proteins
-
Ferric Compounds
-
FhuA protein, E coli
-
Hydroxamic Acids
-
iron (III) hydroxamate
-
phenylacetylene
-
Cobalt
-
Acetylene