The associations between serum adiponectin, leptin, C-reactive protein, insulin, and serum long-chain omega-3 fatty acids in Labrador Retrievers

Renee M Streeter; Angela M Struble; Sabine Mann; Daryl V Nydam; John E Bauer; Marta G Castelhano; Rory J Todhunter; Bethany P Cummings; Joseph J Wakshlag

doi:10.2147/VMRR.S60478

The associations between serum adiponectin, leptin, C-reactive protein, insulin, and serum long-chain omega-3 fatty acids in Labrador Retrievers

Vet Med (Auckl). 2015 Apr 8:6:103-110. doi: 10.2147/VMRR.S60478. eCollection 2015.

Authors

Renee M Streeter¹, Angela M Struble¹, Sabine Mann², Daryl V Nydam², John E Bauer³, Marta G Castelhano¹, Rory J Todhunter¹, Bethany P Cummings⁴, Joseph J Wakshlag¹

Affiliations

¹ Department of Clinical Sciences, jw37@cornell.edu.
² Department of Population Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
³ Department of Clinical Sciences, Texas A&M University, College Station, TX, USA.
⁴ Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.

Abstract

Obesity has been associated with an increased inflammatory response and insulin resistance due to adipose tissue-derived adipokines and increases in C-reactive protein (CRP). Dogs appear to be similar to other species with the exception of adiponectin, which might not be affected by obesity status. Serum long-chain polyunsaturated fatty acid concentrations have been positively and negatively associated with serum adipokines. The aim of the study was to examine the relationship between leptin, CRP, adiponectin, and insulin to body condition score (BCS) and to the long-chain omega-3 fatty acids in serum lipoproteins, including alpha-linolenic acid, eicosapentaenoic acid (EPA), docosapentanenoic acid (DPA), and docosahexaenoic acid (DHA) as a reflection of dietary omega-3 status in the Labrador Retriever. Seventy-seven Labrador Retrievers were evaluated for BCS, percent fasting serum lipoprotein fatty acid concentrations, as well as serum leptin, adiponectin, insulin, and CRP. A multivariable general linear regression model was constructed to examine the association between the dependent variables leptin, CRP, adiponectin, and insulin and the predictor variables of BCS, age, and sex, as well as concentrations of alpha-linolenic acid, EPA, DHA, and DPA. Adiponectin concentration was positively associated with age (P<0.0008), EPA (P=0.027) and negatively associated with DHA (P=0.008). Leptin concentration was positively associated with an increased DHA (P=0.009), BCS (P<0.0001), age (P=0.02), and decreased DPA (P=0.06). Insulin concentration was only associated with BCS (P<0.0001), and no meaningful associations were found for CRP. Longer chain omega-3 fatty acids may play a role in regulating adiponectin concentrations in dogs. However, because insulin concentrations were associated only with BCSs, further examination of the role of adiponectin in canine obesity is warranted. EPA and DPA may reduce the overall inflammatory state in dogs as these omega-3 fatty acids reflect increased adiponectin (increased EPA and decreased DHA) and decreased leptin (decreased DHA and increased DPA).

Keywords: adiponectin; dog; insulin; leptin; obesity; omega-3 fatty acid.