Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H2O2 biosensor applications

Selvakumar Palanisamy; Vijayalakshmi Velusamy; Shih-Wen Chen; Thomas C K Yang; Sridharan Balu; Craig E Banks

doi:10.1016/j.carbpol.2018.10.001

Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H₂O₂ biosensor applications

Carbohydr Polym. 2019 Jan 15:204:152-160. doi: 10.1016/j.carbpol.2018.10.001. Epub 2018 Oct 4.

Authors

Selvakumar Palanisamy¹, Vijayalakshmi Velusamy², Shih-Wen Chen³, Thomas C K Yang⁴, Sridharan Balu³, Craig E Banks⁵

Affiliations

¹ Division of Electrical and Electronic Engineering, School of Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom; Department of Chemical Engineering, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei City, Taiwan, ROC. Electronic address: prmselva@gmail.com.
² Division of Electrical and Electronic Engineering, School of Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom. Electronic address: V.Velusamy@mmu.ac.uk.
³ Department of Chemical Engineering, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei City, Taiwan, ROC.
⁴ Department of Chemical Engineering, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei City, Taiwan, ROC. Electronic address: ckyang@mail.ntut.edu.tw.
⁵ School of Science and the Environment, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom.

PMID: 30366526
DOI: 10.1016/j.carbpol.2018.10.001

Abstract

In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H₂O₂ biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various physio-chemical methods have applied for the characterization of RGO-CMF composite. Cyclic voltammetry results revealed that the hemin/RGO-CMF composite shows a better redox electrochemical behavior than hemin/RGO and hemin/GO-CMF. Under optimized conditions, the hemin/RGO-CMF composite exhibit a linear response to H₂O₂ in the concentration range from 0.06 to 540.6 μM with the lower detection limit of 16 nM. The sensor also can able to detect the H₂O₂ in commercial contact lens solution and milk samples with functional recovery, which authenticates the potential ability in practical sensors.

Keywords: Cellulose microfibers; H(2)O(2) biosensor; Hemin; Peroxidase mimic; Redox electrochemistry; Reduced graphene oxide.