Mutagenesis and Imaging Studies of BMP Signaling Mechanisms in C. elegans

Methods Mol Biol. 2019:1891:51-73. doi: 10.1007/978-1-4939-8904-1_6.

Abstract

C. elegans has played a central role in the elucidation of the TGFβ pathway over the last two decades. This is due to the high conservation of the pathway components and the power of genetic and cell biological approaches applied toward understanding how the pathway signals. In Subheading 3, we detail approaches to study the BMP branch of the TGFβ pathway in C. elegans.

Keywords: Body size; C. elegans; Coelomocytes; Development; Genetic screens; Imaging; Mesoderm; Mutagenesis; Trafficking.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Proteins / genetics*
  • Bone Morphogenetic Proteins / metabolism*
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Genetic Testing
  • Mesoderm / metabolism
  • Molecular Imaging* / methods
  • Mutagenesis*
  • Mutation
  • Signal Transduction*

Substances

  • Bone Morphogenetic Proteins
  • Caenorhabditis elegans Proteins