Measuring the Kinase Activities of the LATS/NDR Protein Kinases

Alexander Hergovich

doi:10.1007/978-1-4939-8910-2_23

Measuring the Kinase Activities of the LATS/NDR Protein Kinases

Methods Mol Biol. 2019:1893:305-317. doi: 10.1007/978-1-4939-8910-2_23.

Author

Alexander Hergovich¹

Affiliation

¹ Cancer Institute, University College London, London, UK. a.hergovich@ucl.ac.uk.

PMID: 30565143
DOI: 10.1007/978-1-4939-8910-2_23

Abstract

The Hippo tissue growth control and regeneration pathway is a main regulator of the YAP/TAZ effectors. In this regard, the LATS/NDR serine/threonine protein kinases can function as central components of the Hippo core module. More specifically, LATS/NDR-mediated phosphorylation of YAP/TAZ on different residues can regulate the subcellular localization and/or stability of YAP/TAZ. Therefore, the assessment of LATS/NDR activities can serve as readout for the activity status of the Hippo pathway. Here, we describe our preferred methodology regarding the measurement of the activities of LATS/NDR kinases.

Keywords: Hippo pathway; Kinase assays; Kinase substrates; LATS1; LATS2; NDR1; NDR2; Protein kinases; STK38; STK38L.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Activation
Enzyme Assays* / methods
Immunoprecipitation
Protein Serine-Threonine Kinases / metabolism*

Substances

Protein Serine-Threonine Kinases

Abstract

Publication types

MeSH terms

Substances

Grants and funding