Background: Coronary Artery Disease (CAD) is one of the most widespread non-communicable diseases. Vitamin Dbinding protein (VDBP) and its genetic poly morphisms have been highlighted as the susceptible components for CAD. The aim of the present study was to examine the association of VDBP single nucleotide poly morphisms (SNPs) - rs7041 and rs4588 - with CAD susceptibility among the Iranian population.
Methods: A total of 143 men with CAD and 145 healthy age-sex matched controls underwent genotyping for the - rs7041 and rs4588 polymorphisms using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Serum level of 25(OH)D was assayed using microplate colorimetric enzyme immunoassay.
Results: We found a significant association between GG genotype (rs7041) and CAD (p=0.02, OR=0.537 95% CI =0.306-0.944). Regarding rs4588 polymorphism, a significant difference was observed in which the CA genotype (p=0.00032, OR=2.578, 95% CI=1.579-4.208) and allele A (P=0.028, OR=1.491, 95% CI=1.043-2.132) were significantly higher in CAD patients compared to controls. In spite of lower serum levels of 25(OH)D in CAD patients, we found no significant association between these SNPs and Vitamin D serum concentrations.
Conclusion: We concluded that VDBP polymorphisms affect the susceptibility to CAD in Iranian men. Therefore, further studies are required to clarify the association of VDBP phenotypes and its serum levels with CAD.
Uvod: Koronarna arterijska bolest (CAD) jedno je od najrasprostranjenijih hroničnih oboljenja. Vitamin D-vezuju}i protein (VDBP) i njegovi genetski polimorfizmi predočeni su kao podložne komponente za CAD. Cilj ove studije bio je da se ispita povezanost između polimorfizama pojedinačnih nukleotida (SNPs) proteina VDBP –rs7041 i rs4588 i podložnosti CAD u populaciji Iranaca.
Metode: Ukupno 143 muškarca sa CAD i 145 zdravih kontrolnih ispitanika odgovarajućeg uzrasta i pola podvrgnuto je genotipizaciji za polimorfizme –rs7041 i rs4588 pomoću metode lančane reakcije polimeraze odnosno polimorfizama dužine restrikcionih fragmenata (PCR-RFLP). Nivo 25(OH)D u serumu određen je pomoću kolorimetrijskog enzimskog imunoeseja na mikroploči.
Rezultati: Otkrili smo značajnu povezanost između genotipa GG (rs7041) i CAD (p=0,02, OR=0,537 95% CI=0,306– 0,944). [to se tiče polimorfizma rs4588, uočena je značajna razlika, pri ~emu su genotip CA (P=0,00032, OR=2,578, 95% CI=1,579–4,208) i alel A (P=0,028, OR=1,491, 95% CI=1,043–2,132) bili značajno viši kod obolelih od CAD u poređenju s kontrolom. Uprkos nižim serumskim nivoima 25(OH)D kod pacijenata sa CAD, nije otkrivena značajna povezanost između ovih polimorfizama i koncentracija vitamina D u serumu.
Zaključak: Zaključili smo da polimorfizmi VDBP utiču na podložnost CAD kod iranskih muškaraca. Dakle, potrebne su dalje studije kako bi se razjasnila povezanost između fenotipova VDBP i njegovih nivoa u serumu i CAD.
Keywords: 25(OH)D; coronary artery disease; polymorphism; vitamin D-binding protein.