Ca2+-Dependent Regulation of TFEB and Lysosomal Function

Methods Mol Biol. 2019:1925:145-155. doi: 10.1007/978-1-4939-9018-4_13.

Abstract

Lysosomes are emerging as calcium store organelles that can modulate various intracellular processes such as the regulation of nutrient signaling through the activation of TFEB, a master gene for lysosomal function, or very specialized functions like lysosomal exocytosis. Here, we describe two different techniques that can be used to study these processes. In the case report, we described two studies where these methodologies allowed us to unravel the role of calcineurin in the dephosphorylation of TFEB as well as the involvement of TFEB in lysosomal exocytosis, respectively.

Keywords: Ca2 +; Calcineurin; Lysosomal exocytosis; Lysosomes; TFEB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / metabolism*
  • Calcineurin / metabolism*
  • Calcium / metabolism*
  • Cations, Divalent / metabolism
  • Cell Culture Techniques / methods
  • Cell Line
  • Exocytosis
  • HeLa Cells
  • Humans
  • Immunoblotting / methods
  • Immunoprecipitation / methods
  • Lysosomes / genetics
  • Lysosomes / metabolism*
  • Mice
  • Phosphorylation
  • Transfection / methods

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Cations, Divalent
  • TFEB protein, human
  • Calcineurin
  • Calcium