Abstract
Pervasive transcription is a feature of the human genome that requires better understanding. Over the last decade or so, RNA species longer than 200 nucleotides-dubbed long non-coding RNA (lncRNAs)-had been found in sense or anti-sense orientation within or outside of genes that encode proteins. Importantly, lncRNA-mediated gene regulation and the elements that control lncRNA expression are a source of fascination among molecular biologists. In vascular biology, a dozen or so lncRNAs had been identified, and progress occurs each day. In this review, we highlighted our laboratories' contribution to the lncRNA field by discussing lessons learned from two lncRNAs in the tyrosine kinase containing immunoglobulin and epidermal growth factor homology1 (Tie1) and delta-like 4 (Dll4) loci. These genes are responsible for basic vascular patterning and pathophysiological remodeling in angiogenesis.
Keywords:
Angiogenesis; CRISPRi; Dll4; Endothelial cells; Morpholinos; Tie1; Vascular; Zebrafish.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Body Patterning
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Calcium-Binding Proteins
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Gene Expression Regulation
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Gene Expression Regulation, Developmental
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Humans
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Intercellular Signaling Peptides and Proteins / genetics*
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Intercellular Signaling Peptides and Proteins / metabolism
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism
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Models, Animal
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Neovascularization, Physiologic / genetics*
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RNA, Long Noncoding / genetics*
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RNA, Long Noncoding / metabolism
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Receptor, TIE-1 / genetics*
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Receptor, TIE-1 / metabolism
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Signal Transduction
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Vascular Remodeling / genetics*
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Zebrafish / genetics
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Zebrafish / metabolism
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Calcium-Binding Proteins
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DLL4 protein, human
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Dll4 protein, zebrafish
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Intercellular Signaling Peptides and Proteins
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Intracellular Signaling Peptides and Proteins
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RNA, Long Noncoding
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Zebrafish Proteins
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Receptor, TIE-1
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tie1 protein, zebrafish