circ_0001730 promotes proliferation and invasion via the miR-326/Wnt7B axis in glioma cells

Epigenomics. 2019 Aug;11(11):1335-1352. doi: 10.2217/epi-2019-0121. Epub 2019 Jul 15.

Abstract

Aim: To study the role of circRNA (circ_0001730) in glioblastoma. Materials & methods: The interaction between circ_0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. Results: The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ_0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/β-catenin pathway via the miR-326/Wnt7B axis. Conclusion: circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.

Keywords: Wnt7B; circRNAs; epithelial to mesenchymal transition; glioblastoma; miR-326; β-catenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Cell Movement
  • Cell Proliferation*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Glioma / genetics
  • Glioma / metabolism
  • Glioma / pathology*
  • Humans
  • Male
  • Mice
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Invasiveness
  • Prognosis
  • RNA, Circular / genetics*
  • Sp1 Transcription Factor / genetics
  • Sp1 Transcription Factor / metabolism
  • Survival Rate
  • Tumor Cells, Cultured
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism*
  • Xenograft Model Antitumor Assays
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • CTNNB1 protein, human
  • MIRN326 microRNA, human
  • MicroRNAs
  • RNA, Circular
  • Sp1 Transcription Factor
  • SP1 protein, human
  • WNT7B protein, human
  • Wnt Proteins
  • beta Catenin