Application of CRISPR-Cas9 Screening Technologies to Study Mitochondrial Biology in Healthy and Disease States

David Sharon; Steven M Chan

doi:10.1007/978-981-13-8367-0_15

Application of CRISPR-Cas9 Screening Technologies to Study Mitochondrial Biology in Healthy and Disease States

Adv Exp Med Biol. 2019:1158:269-277. doi: 10.1007/978-981-13-8367-0_15.

Authors

David Sharon¹, Steven M Chan²

Affiliations

¹ Princess Margaret Cancer Centre, Toronto, ON, Canada.
² Princess Margaret Cancer Centre, Toronto, ON, Canada. Steven.Chan@uhnresearch.ca.

PMID: 31452146
DOI: 10.1007/978-981-13-8367-0_15

Abstract

Mitochondria play a central role in maintaining normal cellular homeostasis as well as contributing to the pathogenesis of numerous disease states. The advent of CRISPR-Cas9 screening technologies has greatly accelerated the study of mitochondrial biology. In this chapter, we review the various CRISPR-Cas9 screening platforms that are currently available and prior studies that leveraged this technology to identify genes involved in mitochondrial biology in both healthy and disease states. In addition, we discuss the challenges associated with current CRISPR-Cas9 platforms and potential solutions to further enhance this promising technology.

Keywords: CRISPR; Cas9; Functional genomics; Knockout screens; Mitochondria; Oxidative phosphorylation; Synthetic lethality.

Publication types

Review

MeSH terms

CRISPR-Cas Systems*
Cell Physiological Phenomena / genetics
Mitochondria* / genetics
Mitochondria* / pathology
Mitochondrial Diseases* / genetics
Research / trends