[Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient]

Bilt Hematol Transfuz. 1978;6(1):13-21.
[Article in Croatian]

Abstract

Peripheral blood lymphocytes of 20 normal volunteers were separated on a discontinuous gradient of Ficoll. The ability of lymphocytes from the fractions to from sponteneous (E) and induced (EAC) rosettes and undergo transformation in the presence of phytohaemagglutinin (PHA) was tested. T-lymphocytes were concentrated in the density of 1,067 g/ml and B-lymphocytes in the density of 1,063 g/ml.

Publication types

  • English Abstract

MeSH terms

  • B-Lymphocytes*
  • Cell Separation / methods*
  • Centrifugation, Density Gradient*
  • Humans
  • Lymphocyte Activation
  • Phytohemagglutinins / pharmacology
  • Rosette Formation
  • T-Lymphocytes*

Substances

  • Phytohemagglutinins