Recombinant DNA technology and DNA sequencing

Essays Biochem. 2019 Oct 16;63(4):457-468. doi: 10.1042/EBC20180039.

Abstract

DNA present in all our cells acts as a template by which cells are built. The human genome project, reading the code of the DNA within our cells, completed in 2003, is undoubtedly one of the great achievements of modern bioscience. Our ability to achieve this and to further understand and manipulate DNA has been tightly linked to our understanding of the bacterial and viral world. Outside of the science, the ability to understand and manipulate this code has far-reaching implications for society. In this article, we explore some of the basic techniques that enable us to read, copy and manipulate DNA sequences alongside a brief consideration of some of the implications for society.

Keywords: CRISPR; DNA sequencing; biochemical techniques and resources.

Publication types

  • Review

MeSH terms

  • Cloning, Molecular / methods
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA, Recombinant / genetics*
  • Electrophoresis, Agar Gel / methods
  • Genetic Testing*
  • Genetic Vectors / genetics
  • Mutation
  • Plants, Genetically Modified / genetics*
  • Polymerase Chain Reaction / methods

Substances

  • DNA, Recombinant
  • DNA