Tooth bioengineering from single cell suspensions

Vassilis Stratoulias; Frederic Michon

doi:10.1016/j.mex.2019.10.009

Tooth bioengineering from single cell suspensions

MethodsX. 2019 Oct 17:6:2429-2438. doi: 10.1016/j.mex.2019.10.009. eCollection 2019.

Authors

Vassilis Stratoulias¹, Frederic Michon^{1

2}

Affiliations

¹ Institute of Biotechnology, Helsinki Institute of Life Science, Developmental Biology Program, University of Helsinki, 00790, Helsinki, Finland.
² Institute for Neurosciences of Montpellier, INSERM UMR1051, University of Montpellier, 34295, Montpellier, France.

Abstract

Recent advances in bioengineering and biomaterials, along with knowledge deriving from the fields of developmental biology and stem cell research, have rendered feasible functional replacement of full organs. Here, we describe the methodology for bioengineering a tooth, starting from embryonic epithelial and mesenchymal single cell suspensions. In addition, we describe the subsequent steps of processing this minute structure for use in applications such as histological examination, immunofluorescence and in situ hybridisation. This methodology can be used for any minute structure that needs to be used in paraffin blocks. •Detailed methodology for reproducible and reliable results•Extra step to ensure single cell populations•Subsequent minute structure processing for histological analysis.

Keywords: Bioengineering; Dental implant; Epithelium; Mesenchyme; Reconstruction; Tooth; Tooth bioengineering.