Three-Way Interactions in an Artificial Community of Bacterial Strains Directly Isolated From the Environment and Their Effect on the System Population Dynamics

Abstract

This work is motivated by previous studies that have analyzed the population ecology of a collection of culturable thermoresistant bacteria, isolated from the Churince lagoon in Cuatro Cienegas, Mexico. In particular, it is aimed at testing a hypothesis from a modeling study, which states that antagonistic and sensitive bacteria co-exist thanks to resistant bacteria that protect sensitive ones by forming physical barriers. We selected three different bacterial strains from the referred collection: one antagonistic, one sensitive, and one resistant, and studied the population dynamics of mixed colonies. Our results show that, although the proposed protective mechanism does not work in this case, the resistant strain confers some kind of protection to sensitive bacteria. Further modeling and experimental results suggest that the presence of resistant bacteria indirectly improves the probability that patches of sensitive bacteria grow in a mixed colony. More precisely, our results suggest that by making antagonistic bacteria produce and secrete an antagonistic substance (with the concomitant metabolic cost and growth rate reduction), resistant bacteria increase the likelihood that sensitive bacteria locally outcompete antagonistic ones.

Keywords: antagonism; mathematical model; microbial ecology; mixed bacterial colonies; population dynamics.