A novel mitochondria-targeted phosphorescence probe for hypochlorite ions detection in living cells

Sili Yi; Zhen Lu; Yandai Lin; Jun Wang; Ziwen Qiao; Rongkai Shen; Jin Zhang; Linxi Hou

doi:10.1016/j.talanta.2019.120516

A novel mitochondria-targeted phosphorescence probe for hypochlorite ions detection in living cells

Talanta. 2020 Mar 1:209:120516. doi: 10.1016/j.talanta.2019.120516. Epub 2019 Oct 31.

Authors

Sili Yi¹, Zhen Lu², Yandai Lin², Jun Wang³, Ziwen Qiao², Rongkai Shen⁴, Jin Zhang⁵, Linxi Hou⁶

Affiliations

¹ College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, PR China; Institute of Food Safety and Environment Monitoring of Photo-catalysis on Energy and Environment College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China.
² College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, PR China.
³ College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China.
⁴ Department of Orthopaedic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350116, PR China.
⁵ College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, PR China. Electronic address: J_Zhang929@fzu.edu.cn.
⁶ College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, PR China. Electronic address: lxhou@fzu.edu.cn.

PMID: 31892012
DOI: 10.1016/j.talanta.2019.120516

Abstract

Monitoring hypochlorite anion (ClO^-) in living cells is particularly meaningful and valuable, because over-exposure of the ClO^- may cause a potential health hazard towards animals and humans. Considering the special structure and properties of the gemini surfactant, a novel amphiphilic gemini-iridium complex Ir[(ppy-iso)₂(bpy-tma₂Br₂)] (Ir-iso) with isoniazide as a recognition site for ClO^- was designed. The Ir-iso possessed an excellent water-solubility as well as a strong ClO^- binding capacity, as revealed from the rapid response of emission signal towards ClO^-. It was worth noting that such probe had a highly-specific selectivity with a low detection limit (20.5 nM) and was suitable in physiological environment. The cell viability assay, cell imaging, and co-location studies further proved that the Ir-iso had little cytotoxicity and was specifically localized in the mitochondria of breast cancer cells, being a promising candidate of chemo-sensor to detect the endogenous ClO^- in living cells.

Keywords: Chemo-sensor; Gemini-iridium complex; Hypochlorite anion; Isoniazide; Mitochondria-targeted.

MeSH terms

Animals
Cell Line, Tumor
Cell Survival / drug effects
Coordination Complexes / chemical synthesis
Coordination Complexes / chemistry*
Coordination Complexes / toxicity
Hypochlorous Acid / analysis*
Iridium / chemistry
Isoniazid / analogs & derivatives*
Isoniazid / chemical synthesis
Isoniazid / toxicity
Limit of Detection
Luminescent Agents / chemical synthesis
Luminescent Agents / chemistry*
Luminescent Agents / toxicity
Luminescent Measurements / methods
Mice
Microscopy, Confocal / methods
Mitochondria / metabolism*

Substances

Coordination Complexes
Luminescent Agents
Iridium
Hypochlorous Acid
Isoniazid