The gene arrangement and phylogeny using mitochondrial genomes in spiders (Arachnida: Araneae)

Abstract

The complete mitochondrial genome (mitogenome) of Cheiracanthium triviale was sequenced for the first time. The 14,595 bp C. triviale mitogenome contained 37 genes (13 protein coding genes, 2 ribosomal RNAs, 22 transfer RNAs) and one control region. The mitogenome of Dysdera silvatica which was available at NCBI GenBank was annotated. The mitogenome of C. triviale was compared with 43 previously sequenced spider species to observe the gene arrangements, control region and phylogeny. TreeREx analysis identified 19 mitochondrial gene rearrangements (11 transposition, 6 inversion, 2 inverse transposition) in spiders as compared with the putative ancestral gene order and lead to form new gene boundaries: trnQ-trnA, trnA-trnM for Loxosceles similis; nad3-trnS1, trnE-trnL2, trnL2-trnA, trnN-trnF for Agelena silvatica; trnN-trnE, trnE-trnA, trnR-trnF, nad4L-trnW, trnW-trnP for Carrhotus xanthogramma; trnQ-trnW, trnW-trnG, trnG-trnM for Tetragnatha nitens. Our study revealed that the gene rearrangement in spiders with putative ancestor is accelerated in Araneomorphae as compared to Mygalomorphae. Phylogenetic analysis of spiders using mitochondrial sequence data supports the monophyly of two infraorders, and sister relationship of Cheiracanthiidae with Selenopidae and Salticidae. The systematic position of the Cheiracanthium species always a controversial issue as this taxa was placed in different families by different authors.

Keywords: Gene arrangement; Mitogenome; Phylogeny.