Abstract
Which neural circuits undergo synaptic changes when an animal learns? Although it is widely accepted that changes in synaptic strength underlie many forms of learning and memory, it remains challenging to connect changes in synaptic strength at specific neural pathways to specific behaviors and memories. Here we introduce SYNPLA (synaptic proximity ligation assay), a synapse-specific, high-throughput, and potentially brain-wide method capable of detecting circuit-specific learning-induced synaptic plasticity.
Keywords:
GluA1; defense conditioning; fear conditioning; proximity ligation assay; synaptic potentiation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Auditory Cortex / chemistry
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Auditory Cortex / cytology
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Auditory Cortex / metabolism
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Cells, Cultured
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Conditioning, Psychological / physiology
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Geniculate Bodies / chemistry
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Geniculate Bodies / cytology
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Geniculate Bodies / metabolism
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High-Throughput Screening Assays / methods*
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Hippocampus / chemistry
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Hippocampus / cytology
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Hippocampus / metabolism
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Learning / physiology*
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Mice
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / metabolism
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Neuronal Plasticity / physiology*
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Protein Interaction Mapping / methods*
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Rats
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Synapses* / chemistry
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Synapses* / metabolism