Single-cell analyses uncover granularity of muscle stem cells

John Saber; Alexander Y T Lin; Michael A Rudnicki

doi:10.12688/f1000research.20856.1

Single-cell analyses uncover granularity of muscle stem cells

F1000Res. 2020 Jan 21:9:F1000 Faculty Rev-31. doi: 10.12688/f1000research.20856.1. eCollection 2020.

Authors

John Saber^#^{1

2}, Alexander Y T Lin^#^{1

2}, Michael A Rudnicki^{1

2}

Affiliations

¹ Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada.
² Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada.

^# Contributed equally.

Abstract

Satellite cells are the main muscle-resident cells responsible for muscle regeneration. Much research has described this population as being heterogeneous, but little is known about the different roles each subpopulation plays. Recent advances in the field have utilized the power of single-cell analysis to better describe and functionally characterize subpopulations of satellite cells as well as other cell groups comprising the muscle tissue. Furthermore, emerging technologies are opening the door to answering as-yet-unresolved questions pertaining to satellite cell heterogeneity and cell fate decisions.

Keywords: cell fate; heterogeneity; satellite cells; single-cell analysis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell Differentiation
Humans
Muscle Development
Muscle, Skeletal / cytology*
Satellite Cells, Skeletal Muscle / cytology*
Single-Cell Analysis*

Abstract

Publication types

MeSH terms

Grants and funding