The endogenous muscarinic acetylcholine receptor in Xenopus oocytes is of the M3 subtype

L A Van Wezenbeek; J A Tonnaer; G S Ruigt

doi:10.1016/0014-2999(88)90551-1

The endogenous muscarinic acetylcholine receptor in Xenopus oocytes is of the M3 subtype

Eur J Pharmacol. 1988 Jul 14;151(3):497-500. doi: 10.1016/0014-2999(88)90551-1.

Authors

L A Van Wezenbeek¹, J A Tonnaer, G S Ruigt

Affiliation

¹ CNS Pharmacology R&D Laboratory, Organon Int. B.V., Oss, The Netherlands.

PMID: 3215274
DOI: 10.1016/0014-2999(88)90551-1

Abstract

The muscarinic acetylcholine receptor of the Xenopus oocyte was characterized electrophysiologically. Iontophoretic responses to acetylcholine were inhibited by the muscarinic antagonists 4-DAMP, pirenzepine and AF-DX 116 at respective IC50 values of 7 nM, 15 microM and 2 microM. This antagonist sensitivity order indicates that the receptor is of the M3 muscarinic subtype.

MeSH terms

Acetylcholine / pharmacology
Animals
Female
In Vitro Techniques
Membrane Potentials / drug effects
Oocytes / metabolism*
Piperidines / pharmacology
Pirenzepine / analogs & derivatives
Pirenzepine / pharmacology
Receptors, Cholinergic / metabolism*
Xenopus laevis

Substances

Piperidines
Receptors, Cholinergic
Pirenzepine
4-diphenylacetoxy-1,1-dimethylpiperidinium
Acetylcholine
otenzepad