Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery

Chenzhen Zhang; Tengfei He; Armin Vedadghavami; Ambika G Bajpayee

doi:10.1016/j.mex.2020.100882

Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery

MethodsX. 2020 Apr 22:7:100882. doi: 10.1016/j.mex.2020.100882. eCollection 2020.

Authors

Chenzhen Zhang¹, Tengfei He¹, Armin Vedadghavami¹, Ambika G Bajpayee^{1

2}

Affiliations

¹ Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA.
² Department of Mechanical Engineering, Northeastern University, Boston, MA, 02115, USA.

Abstract

Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues. We use Avidin-biotin technology that gives the flexibility for conjugating biotinylated Dexamethasone to mAv by simple mixing at room temperature. We also describe methods to control hydrolysis rates of ester linkers to enable sustained (and tunable) drug release rates in therapeutic doses.•Multi-arm structure provides multiple sites for covalent conjugation of drugs•Use of Avidin-biotin reaction gives multi-arm nano-construct a modular design enabling conjugation and delivery of similar sized biotinylated drugs.

Keywords: Avidin; Biotin; Drug delivery; Ester linkers; Negatively charged tissues; PEGs.

Grants and funding

R03 EB025903/EB/NIBIB NIH HHS/United States