Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development

PLoS One. 2020 May 26;15(5):e0233394. doi: 10.1371/journal.pone.0233394. eCollection 2020.

Abstract

Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9-/-animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Chromatin / metabolism
  • Chromatin Assembly and Disassembly
  • DNA Helicases / genetics*
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Gene Knockout Techniques
  • Germ-Line Mutation
  • Humans
  • K562 Cells
  • Mice
  • Mouse Embryonic Stem Cells / cytology
  • Trans-Activators / genetics*

Substances

  • Chromatin
  • Trans-Activators
  • DNA Helicases
  • CHD9 protein, human
  • Chd9 protein, mouse

Grants and funding

This project was supported by a Queen Wilhelmina Research Prize from the Dutch Cancer Society assigned to A.B. https://www.kwf.nl/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.