Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

Yu-Shui Ma; Kai-Jian Chu; Chang-Chun Ling; Ting-Miao Wu; Xu-Chao Zhu; Ji-Bin Liu; Fei Yu; Zhi-Zhen Li; Jing-Han Wang; Qing-Xiang Gao; Bin Yi; Hui-Min Wang; Li-Peng Gu; Liu Li; Lin-Lin Tian; Yi Shi; Xiao-Qing Jiang; Da Fu; Xiong-Wen Zhang

doi:10.1016/j.omtn.2020.05.032

Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

Mol Ther Nucleic Acids. 2020 Sep 4:21:229-241. doi: 10.1016/j.omtn.2020.05.032. Epub 2020 Jun 1.

Authors

Yu-Shui Ma¹, Kai-Jian Chu², Chang-Chun Ling³, Ting-Miao Wu⁴, Xu-Chao Zhu⁵, Ji-Bin Liu⁶, Fei Yu⁵, Zhi-Zhen Li², Jing-Han Wang², Qing-Xiang Gao², Bin Yi², Hui-Min Wang⁵, Li-Peng Gu⁵, Liu Li⁵, Lin-Lin Tian⁵, Yi Shi⁵, Xiao-Qing Jiang², Da Fu⁷, Xiong-Wen Zhang⁸

Affiliations

¹ Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China; Central Laboratory for Medical Research, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
² Department of Biliary Tract Surgery I, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China.
³ Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
⁴ Department of Radiology, The Forth Affiliated Hospital of Anhui Medical University, Hefei 230012, China.
⁵ Central Laboratory for Medical Research, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
⁶ Cancer Institute, Nantong Tumor Hospital, Nantong 226631, China.
⁷ Central Laboratory for Medical Research, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China; Department of Radiology, The Forth Affiliated Hospital of Anhui Medical University, Hefei 230012, China. Electronic address: fu800da900@126.com.
⁸ Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China. Electronic address: xwzhang@sat.ecnu.edu.cn.

Abstract

Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to be investigated. In this study, we demonstrated that OIP5-AS1 was upregulated in LIHC tissue specimens and its overexpression was associated with the poor survival of patients with LIHC. Furthermore, loss-of function experiments indicated that OIP5-AS1 promoted cell proliferation and inhibited cell apoptosis both in vitro and in vivo. Moreover, binding sites between OIP5-AS1 and hsa-miR-26a-3p as well as between hsa-miR-26a-3p and EPHA2 were confirmed by luciferase assays. Finally, a rescue assay was performed to prove the effect of the OIP5-AS1/hsa-miR-26a-3p/EPHA2 axis on LIHC cell biological behaviors. Based on all of the above findings, our results suggested that OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis.

Keywords: EPHA2; LIHC; OIP5-AS1; hsa-miR-26a-3p; prognosis.

Publication types

Retracted Publication