Improving spatial resolution of a LTQ Orbitrap MALDI source

Raul Montero; Beatriz Abad-García; Jone Garate; Lucía Martín-Saiz; Gwendolyn Barceló-Coblijn; Jose A Fernandez

doi:10.1021/jasms.0c00133

Improving spatial resolution of a LTQ Orbitrap MALDI source

J Am Soc Mass Spectrom. 2020 Jul 14. doi: 10.1021/jasms.0c00133. Online ahead of print.

Authors

Raul Montero, Beatriz Abad-García, Jone Garate, Lucía Martín-Saiz, Gwendolyn Barceló-Coblijn, Jose A Fernandez

PMID: 32662650
DOI: 10.1021/jasms.0c00133

Abstract

Here we present a simple and cost effective procedure to improve the spatial resolution of the commercial MALDI source of a LTQ Orbitrap. Based in spatial filtering techniques, we demonstrate that, with minimal modifications of the original set up, the system resolution can be pushed forward to <10 μm. The improved system performance is demonstrated by means of MALDI imaging of human colon biopsies.