Authentication, phytochemical characterization and anti-bacterial activity of twoArtemisiaspecies

Meng-Ting Yang; Tien-Fen Kuo; Kuo-Fang Chung; Yu-Chuan Liang; Chu-Wen Yang; Chih-Yu Lin; Ching-Shan Feng; Zeng-Weng Chen; Tsung-Han Lee; Chiao-Ling Hsiao; Wen-Chin Yang

doi:10.1016/j.foodchem.2020.127458

Authentication, phytochemical characterization and anti-bacterial activity of twoArtemisiaspecies

Food Chem. 2020 Dec 15:333:127458. doi: 10.1016/j.foodchem.2020.127458. Epub 2020 Jul 4.

Authors

Affiliations

¹ Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan; Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung Hsing University and Academia Sinica, Taipei, Taiwan; Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
² Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
³ Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.
⁴ Department of Microbiology, Soochow University, Taipei, Taiwan.
⁵ Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan; Graduate Program of Marine Biotechnology, National Taiwan Ocean University and Academia Sinica, Keelung, Taiwan.
⁶ Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu, Taiwan.
⁷ Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
⁸ Department of Agronomy, National Chung Hsing University, Taichung, Taiwan; Crop Science Division, Taiwan Agricultural Research Institute, Taichung, Taiwan. Electronic address: clhsiao@tari.gov.tw.
⁹ Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan; Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung Hsing University and Academia Sinica, Taipei, Taiwan; Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan; Graduate Program of Marine Biotechnology, National Taiwan Ocean University and Academia Sinica, Keelung, Taiwan; Department of Institute of Biotechnology, National Taiwan University, Taipei, Taiwan; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan. Electronic address: wcyang@gate.sinica.edu.tw.

PMID: 32673952
DOI: 10.1016/j.foodchem.2020.127458

Abstract

Artemisia species are aromatic herbs used as food and/or ethnomedicine worldwide; however, the use of these plants is often impeded by misidentification. Here, molecular and chemotaxonomic approaches were combined to assist in the morphology-based authentication of Artemisia species, and Artemisia indica and Artemisia argyi were identified. The plant extracts and compounds obtained from these species, 1,8-cineole, carveol, α-elemene, α-farnesene, methyl linolenate, diisooctyl phthalate inhibited the growth of food-borne harmful bacteria. Mechanistic studies showed that the extract and active compounds of A. indica killed Gram-negative and -positive bacteria via destruction of the bacterial membrane. Finally, in vivo data demonstrated that A. indica protected against bacterial infection in mice as evidenced by survival rate, bacterial load in organs, gut pathology, diarrhea, body weight, food consumption, stool weight, and pathology score. A. indica and its active compounds have potential for use as food supplements for food-borne bacterial diseases and thus improve human health.

Keywords: 1,8-Cineole (PubChem CID: 2758); Artemisia; Carveol (PubChem CID: 7438); Diarrhea; Diisooctyl phthalate (PubChem CID: 33934); Food-borne bacteria; Methyl linolenate (PubChem CID: 5319706); Plant authentication; α-Elemene (PubChem CID: 80048); α-Farnesene (PubChem CID: 5281516).

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Artemisia / chemistry*
Bacterial Load
Diarrhea / drug therapy
Diarrhea / microbiology
Female
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Mice, Inbred BALB C
Microbial Sensitivity Tests
Phytochemicals / analysis*
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Plants, Medicinal / chemistry
Salmonella Food Poisoning / drug therapy
Salmonella Food Poisoning / mortality
Taiwan

Substances

Anti-Bacterial Agents
Phytochemicals
Plant Extracts