High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

Nat Methods. 2020 Sep;17(9):913-916. doi: 10.1038/s41592-020-0882-0. Epub 2020 Aug 3.

Abstract

Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Caenorhabditis elegans / microbiology
  • Caenorhabditis elegans / physiology*
  • Elasticity
  • Escherichia coli
  • Microscopy, Confocal / methods*
  • Scattering, Radiation*
  • Viscosity