Draft genome sequence of plastic degrading Bacillus sp. AIIW2 isolated from the Arabian ocean

J Basic Microbiol. 2021 Jan;61(1):37-44. doi: 10.1002/jobm.202000416. Epub 2020 Oct 1.

Abstract

The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was found to utilize plastic as a carbon source. The Bacillus sonorensis SRCM101395 was used as a reference genome for mapping the reads. The genome size of strain AIIW2 was approximately 4.4 Mb and composed of 4737 coding sequences with 45.7% G + C contents. The whole genome comparison of strain AIIW2 with three closest Bacillus strains showed strain specificity, the 16S ribosomal RNA sequence shows 99.93% similarity with Bacillus paralicheniformis KJ-16T (KY694465). This genome data would provide the genetic basis in developing plastic bioremediation approaches and discover the enzymes pertinent in the biodegradation processes.

Keywords: biodegradation; draft genome; marine bacteria; plastic.

MeSH terms

  • Bacillus / classification
  • Bacillus / genetics*
  • Bacillus / isolation & purification
  • Bacillus / metabolism
  • Biodegradation, Environmental
  • DNA, Bacterial / genetics
  • Genes, Bacterial / genetics
  • Genome, Bacterial / genetics*
  • Oceans and Seas
  • Phylogeny
  • Plastics / metabolism*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA, Bacterial
  • Plastics
  • RNA, Ribosomal, 16S