Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Olga E Valuyskikh; Ludmila V Teteryuk; Yana I Pylina; Oleg E Sushentsov; Nikita A Martynenko; Dmitry M Shadrin

doi:10.3897/phytokeys.162.53361

Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

PhytoKeys. 2020 Oct 9:162:113-130. doi: 10.3897/phytokeys.162.53361. eCollection 2020.

Authors

Olga E Valuyskikh¹, Ludmila V Teteryuk¹, Yana I Pylina¹, Oleg E Sushentsov², Nikita A Martynenko³, Dmitry M Shadrin¹

Affiliations

¹ Institute of Biology of Komi Scientific Center of Ural Branch of Russian Academy of Sciences, Kommunisticheskaya 28, Syktyvkar, 167982, Russia Komi Scientific Center, Ural Branch of Russian Academy of Sciences Syktyvkar Russia.
² Botanical Garden of Ural Branch of Russian Academy of Sciences, 8 Marta 202a, Yekaterinburg, 620144, Russia Botanical Garden, Ural Branch of Russian Academy of Sciences Yekaterinburg Russia.
³ K.A. Timiryazev Institute of Plant Physiology of Russian Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russia K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences Moscow Russia.

Abstract

We studied the allopatric complex Pulsatilla patens (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of P. patens with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (rbcL and matK) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (rbcL+matK+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa P. uralensis and P. patents s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of P. uralensis.

Keywords: Ranunculales; DNA barcode; ITS2; matK; molecular phylogeny; rbcL; taxonomy.

Olga E. Valuyskikh, Ludmila V. Teteryuk, Yana I. Pylina, Oleg E. Sushentsov, Nikita A. Martynenko, Dmitry M. Shadrin.