Genetically Engineered Mouse Model in Ewing Sarcoma

Methods Mol Biol. 2021:2226:183-189. doi: 10.1007/978-1-0716-1020-6_14.

Abstract

Modeling Ewing sarcoma is challenging, since overexpression of EWS-FLI1 induces apoptosis and is not sufficient for tumor induction. It is therefore important to obtain the cell-of-origin of Ewing sarcoma that is tolerant of EWS-FLI1 expression. Here we describe the generation of the EWS-FLI1-expressing mouse model for Ewing sarcoma by selecting embryonic chondrogenic progenitor, eSZ cells that contain Ewing sarcoma precursors.

Keywords: EWS-FLI1; Embryonic superficial zone; Ewing sarcoma; Mouse model; Retrovirus-mediated gene transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers, Tumor
  • Biopsy
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / pathology*
  • Cell Line, Tumor
  • Disease Models, Animal*
  • Humans
  • Mice
  • Mice, Transgenic*
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Protein c-fli-1
  • RNA-Binding Protein EWS
  • Sarcoma, Ewing / genetics*
  • Sarcoma, Ewing / pathology*

Substances

  • Biomarkers, Tumor
  • EWS-FLI fusion protein
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Protein c-fli-1
  • RNA-Binding Protein EWS