Modeling Ewing sarcoma is challenging, since overexpression of EWS-FLI1 induces apoptosis and is not sufficient for tumor induction. It is therefore important to obtain the cell-of-origin of Ewing sarcoma that is tolerant of EWS-FLI1 expression. Here we describe the generation of the EWS-FLI1-expressing mouse model for Ewing sarcoma by selecting embryonic chondrogenic progenitor, eSZ cells that contain Ewing sarcoma precursors.
Keywords: EWS-FLI1; Embryonic superficial zone; Ewing sarcoma; Mouse model; Retrovirus-mediated gene transfer.