Real-time monitoring of caspase-3/8 activity by self-assembling nanofiber probes in living cells

Li-Song Zhang; Hong-Lei Xu; Ying Xia; Jian-Peng Bi; Chuan-Zeng Zhang; Zhen Xi; Lu-Yuan Li; Zhi-Song Zhang

doi:10.1039/d0cc07821b

Real-time monitoring of caspase-3/8 activity by self-assembling nanofiber probes in living cells

Chem Commun (Camb). 2021 Jan 18;57(6):797-800. doi: 10.1039/d0cc07821b. Epub 2020 Dec 23.

Authors

Li-Song Zhang¹, Hong-Lei Xu, Ying Xia, Jian-Peng Bi, Chuan-Zeng Zhang, Zhen Xi, Lu-Yuan Li, Zhi-Song Zhang

Affiliation

¹ State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, NanKai University, Tianjin 300350, China. zzs@nankai.edu.cn.

PMID: 33355564
DOI: 10.1039/d0cc07821b

Abstract

Caspase-3/8 are key members of the cysteine-aspartyl protease family with pivotal roles in apoptosis. We have designed and synthesized self-assembling probes, Nap-GFFpYDEVD-AFC and Nap-GFFpYIETD-AFC, with fluorescence 'turn-on' properties for real-time monitoring of Caspase-3/8 activity in living cells.

MeSH terms

Caspase 3 / analysis*
Caspase 3 / metabolism
Caspase 8 / analysis*
Caspase 8 / metabolism
Enzyme Assays / methods*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Microscopy, Confocal
Molecular Structure
Nanofibers / chemistry*
Time Factors

Substances

Fluorescent Dyes
CASP3 protein, human
CASP8 protein, human
Caspase 3
Caspase 8