Isolation of tumor-resident CD8+ T cells from human lung tumors

Stéphanie Corgnac; Yann Lecluse; Fathia Mami-Chouaib

doi:10.1016/j.xpro.2020.100267

Isolation of tumor-resident CD8⁺ T cells from human lung tumors

STAR Protoc. 2021 Jan 12;2(1):100267. doi: 10.1016/j.xpro.2020.100267. eCollection 2021 Mar 19.

Authors

Stéphanie Corgnac¹, Yann Lecluse², Fathia Mami-Chouaib¹

Affiliations

¹ INSERM UMR 1186, Integrative Tumor Immunology and Immunotherapy, Gustave Roussy, Fac. de Médecine - Univ. Paris-Sud, Université Paris-Saclay, Villejuif 94805, France.
² CNRS ums3655 INSERM us23 Gustave Roussy Cancer Campus, Villejuif 94805, France.

Abstract

CD103⁺CD8⁺ tumor-resident memory T cells (T_RM) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8⁺ T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8⁺ T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T_RM cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).

Keywords: Cancer; Cell isolation; Flow cytometry/mass cytometry; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD8-Positive T-Lymphocytes / immunology*
Cell Separation*
Humans
Lung Neoplasms / immunology*
Lymphocytes, Tumor-Infiltrating / immunology*
Memory T Cells / immunology*
Tumor Microenvironment / immunology*