DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids

Akimitsu Okamoto

doi:10.1007/978-981-33-6064-8_17

DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids

Adv Exp Med Biol. 2021:1310:475-493. doi: 10.1007/978-981-33-6064-8_17.

Author

Akimitsu Okamoto¹

Affiliation

¹ Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan. okamoto@chembio.t.u-tokyo.ac.jp.

PMID: 33834446
DOI: 10.1007/978-981-33-6064-8_17

Abstract

Fluorescence imaging of nucleic acids is a key technology needed to understand gene expression and the resulting changes in cellular function. This chapter focuses on sequence-specific fluorescence imaging of nucleic acids in cells using fluorescent nucleic acids. The design and preparation of fluorescent nucleic acids and their application to fluorescence imaging of intracellular nucleic acids are introduced.

Keywords: Cell imaging; DNA; Fluorescence; RNA; Synthetic nucleic acid; Thiazole orange.

MeSH terms

DNA / genetics
Fluorescence
Fluorescent Dyes
Nucleic Acids* / genetics
Optical Imaging
RNA* / genetics
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Nucleic Acids
RNA
DNA