D-Galactose Induces Chronic Oxidative Stress and Alters Gut Microbiota in Weaned Piglets

Hui Han; Zemin Liu; Jie Yin; Jing Gao; Liuqin He; Chenyu Wang; Ruoxin Hou; Xingguo He; Guoqiang Wang; Tiejun Li; Yulong Yin

doi:10.3389/fphys.2021.634283

D-Galactose Induces Chronic Oxidative Stress and Alters Gut Microbiota in Weaned Piglets

Front Physiol. 2021 Apr 8:12:634283. doi: 10.3389/fphys.2021.634283. eCollection 2021.

Authors

Hui Han^{1

2}, Zemin Liu^{1

3}, Jie Yin⁴, Jing Gao^{5

6}, Liuqin He^{1

7}, Chenyu Wang^{1

3}, Ruoxin Hou^{1

3}, Xingguo He⁸, Guoqiang Wang⁸, Tiejun Li^{1

3}, Yulong Yin^{1

3

7}

Affiliations

¹ Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
² State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
³ College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
⁴ College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
⁵ Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Changsha, China.
⁶ National Engineering Research Center for Oil Tea Camellia, Changsha, China.
⁷ Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.
⁸ Changsha Lvye Bio-Technology Co., Ltd., Changsha, China.

Abstract

Oxidative stress commonly occurs in pig production, which can severely damage the intestinal function of weaned piglets. This study was conducted to investigate the effects of D-galactose with different levels used to induce chronic oxidative stress on growth performance, intestinal morphology and gut microbiota in weaned piglets. The results showed that addition of 10 and 20 g/kg BW D-galactose reduced average daily gain and average daily feed intake from the first to the third week. 10 g/kg BW D-galactose increased the concentration of serum MDA at the second and third week. 10 g/kg BW D-galactose significantly influenced the jejunal and ileal expressions of GPx1, CAT1, and MnSOD. The results of 16S rRNA sequencing showed that compared with the control, 10 and 20 g/kg BW D-galactose significantly decreased the relative abundance of Tenericutes, Erysipelotrichia, Erysipelotrichales, and Erysipelotrichaceae, while increased the relative abundance of Negativicutes, Selenomonnadales, and Veillonellaceae. The results indicated that treatment with 10 g/kg BW/day D-galactose for 3 weeks could induce chronic oxidative stress, reduce the growth performance and alter gut microbiota in weaned piglets.

Keywords: D-galactose; chronic oxidative stress; growth performance; gut microbiota; weaned piglets.