Rapid Detection of Transposon-Induced Genome Rearrangements

Sharu Paul Sharma; Thomas Peterson

doi:10.1007/978-1-0716-1134-0_13

Rapid Detection of Transposon-Induced Genome Rearrangements

Methods Mol Biol. 2021:2250:131-139. doi: 10.1007/978-1-0716-1134-0_13.

Authors

Sharu Paul Sharma¹, Thomas Peterson^{2

3}

Affiliations

¹ Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
² Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA. thomasp@iastate.edu.
³ Department of Agronomy, Iowa State University, Ames, IA, USA. thomasp@iastate.edu.

PMID: 33900600
DOI: 10.1007/978-1-0716-1134-0_13

Abstract

This chapter details the techniques used to detect transposon-induced genome rearrangements. Here, we describe a rapid DNA isolation technique, PCR amplification, and a novel High Efficiency Agarose Gel Electrophoresis Method (HEA-GEM).

Keywords: High efficiency agarose gel electrophoresis; Large population size; PCR; Plate DNA extraction; Rapid DNA isolation.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA Transposable Elements*
DNA, Plant / genetics*
Electrophoresis, Agar Gel
Mutagenesis, Insertional
Oryza / genetics*
Polymerase Chain Reaction

Substances

DNA Transposable Elements
DNA, Plant