Complete mitochondrial genome of the Pacific limpet Cellana nigrolineata (Gastropoda: Patellogastropoda) determined by shotgun sequencing using the Illumina NGS platform

Satoko Nakashima; Moe Shimizu; Kazuki Hirota; Shimpei F Hiruta; Nakaji Nagisa; Toshihiko Fujita; Takenori Sasaki; Davin H E Setiamarga

doi:10.1080/23802359.2021.1934579

Complete mitochondrial genome of the Pacific limpet Cellana nigrolineata (Gastropoda: Patellogastropoda) determined by shotgun sequencing using the Illumina NGS platform

Mitochondrial DNA B Resour. 2021 Jun 3;6(7):1857-1859. doi: 10.1080/23802359.2021.1934579.

Authors

Satoko Nakashima¹, Moe Shimizu¹, Kazuki Hirota^{1

2}, Shimpei F Hiruta³, Nakaji Nagisa^{1

4}, Toshihiko Fujita³, Takenori Sasaki², Davin H E Setiamarga^{1

2}

Affiliations

¹ National Institute of Technology (KOSEN), Wakayama College, Wakayama, Japan.
² The University Museum, The University of Tokyo, Tokyo, Japan.
³ National Museum of Nature and Science, Ibaraki, Japan.
⁴ Graduate School of Science, The University of Tokyo, Tokyo, Japan.

Abstract

The Pacific limpet Cellana nigrolineata is one of the most commonly found limpets in the intertidal shores of Japan. Here, we report the full mitogenome sequence of an individual specimen of the species, which was collected from the intertidal rocky beach in the Nada beach of Gobo City, Wakayama, Japan (33.8316 N, 135.1751 E), in 2018. The sequence was determined by the shotgun sequencing method using the NGS Illumina MiSeq platform. The genomic structure of C. nigrolineata is the same as the previously reported congener, C. radiata, which shows a representative Nacellidae and metazoan mitogenomic structures. The mitogenome has all of its 37 genes included in its 16,153 bp, with one control region located between the tRNA-Cys and tRNA-Gly genes. In order to clarify the phylogenetic position of C. nigrolineata in Gastropoda, a data set including the mitogenomes of 10 patellogastropods, 10 non-patellogastropod gastropods, and four outgroups were used in maximum likelihood inferences. Although with some exceptions, the resulting phylogeny supported the monophylies of traditionally accepted gastropod subclasses, and thus confirms the position of C. nigrolineata in Patellogastropoda.

Keywords: Cellana nigrolineata; Nada Coast; Patellogastropoda; intertidal; mitogenome shotgun sequencing.

Grants and funding

This study was partially supported by the National Institute of Technology, Wakayama College Competitive Internal Research Grant for Education 2018, and FY2019 JSPS KAKENHI Grant-in-Aid for Scientific Research (C) Grant [No. 19K12424; to DHES and TS], and JSPS KAKENHI for Challenging Research (Exploratory) Grant [No. 19K21646; to TS, TF, and DHES].