Placenta inflammation is closely associated with gestational diabetes mellitus

Xue Pan; Xin Jin; Jun Wang; Qing Hu; Bing Dai

Placenta inflammation is closely associated with gestational diabetes mellitus

Am J Transl Res. 2021 May 15;13(5):4068-4079. eCollection 2021.

Authors

Xue Pan¹, Xin Jin¹, Jun Wang¹, Qing Hu¹, Bing Dai²

Affiliations

¹ Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang, China.
² Department of Pediatric, Shengjing Hospital of China Medical University Shenyang, China.

PMID: 34149999
PMCID: PMC8205654

Abstract

Objective: To investigate the potential role of placenta inflammation in gestational diabetes mellitus (GDM) and construct a model for the diagnosis of GDM.

Methods: In this study, transcriptome-wide profiling datasets, GSE70493 and GSE128381 were downloaded from Gene Expression Omnibus (GEO) database. Significant immune-related genes were identified separately to be the biomarkers for the diagnosis of GDM by using random forest model (RF), support vector machine model (SVM), and generalized linear model (GLM).

Results: RF was the best model and was used to select the four key immune-related genes (FABP4, DKK1, CXCL10, and IL1RL1) to diagnose GDM. A nomogram model was constructed to predict GDM based on the four key immune-related genes by using "rms" package. The relative proportion of 22 immune cell types were calculated by using CIBERSORT algorithm. Higher M1 macrophage ratio and lower M2 macrophage ratio in GDM placenta compared to normal patients were observed.

Conclusions: This study provides clues that inflammation was correlated with GDM and suggests inflammation may be the cause and also the potential targets of GDM.

Keywords: GDM; consensus clustering; immune-related genes; inflammation; nomogram; placenta.