ADAMTS1 is regulated by the EP4 receptor in the zebrafish ovary

Abstract

Prostaglandins (PGs) are a class of fatty-acid derived hormones that are essential in ovulation of teleosts, but their exact role remains unknown. One putative target of PGs in ovulation is regulation of the expression of members of the A Disintegrin and Metalloproteinase with Thrombospondin motifs (ADAMTS) family, which are implicated in follicular rupture. This study investigated the regulation of ADAMTS, other proteases, and their inhibitors in response to treatment with PGE₂ or PGF_2α. Four members of the ADAMTS family, ADAMTS1, ADAMTS5, ADAMTS9, and ADAMTS16 were shown to be expressed in the ovary of zebrafish, but only adamts1 was upregulated in full-grown follicles following treatment with PGE₂. Inhibitors of the PG receptors EP1 and EP2 had no effect on PGE₂-stimulated adamts1 expression, while treatment of full-grown follicles with both PGE₂ and GW627368x, an inhibitor of EP4 function, prevented the PGE₂-induced increase in adamts1 expression. Treatment of full-grown follicles with the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P) in vitro had no effect on the expression of adamts1 mRNA. These findings suggest that expression of ADAMTS1 in zebrafish ovarian follicles is regulated by the prostaglandin PGE₂ via the EP4 series prostaglandin receptor.

Keywords: ADAMTS1; Ovarian follicle; Ovulation; Prostaglandin; Reproduction; Zebrafish.