Altered plasma levels of lysophospholipids in response to adrenalectomy of rats

Toshihiko Tsutsumi; Masaki Ino; Yoshibumi Shimizu; Kohei Kawabata; Hiroyuki Nishi; Akira Tokumura

doi:10.1016/j.prostaglandins.2021.106579

Altered plasma levels of lysophospholipids in response to adrenalectomy of rats

Prostaglandins Other Lipid Mediat. 2021 Oct:156:106579. doi: 10.1016/j.prostaglandins.2021.106579. Epub 2021 Jul 7.

Authors

Toshihiko Tsutsumi¹, Masaki Ino², Yoshibumi Shimizu², Kohei Kawabata³, Hiroyuki Nishi³, Akira Tokumura⁴

Affiliations

¹ Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshinomachi, Nobeoka, 882-8508, Japan.
² Graduate School of Biomedical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima, 770-8505, Japan.
³ Department of Pharmacy, Yasuda Women's University, 13-6-1 Yasuhigashi, Asaminamiku, Hiroshima, 731-0153, Japan.
⁴ Graduate School of Biomedical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima, 770-8505, Japan; Department of Pharmacy, Yasuda Women's University, 13-6-1 Yasuhigashi, Asaminamiku, Hiroshima, 731-0153, Japan. Electronic address: tokumura@yasuda-u.ac.jp.

PMID: 34245896
DOI: 10.1016/j.prostaglandins.2021.106579

Abstract

The aim of this study was to investigate effects of reduced stress hormone by adrenalectomy on rat plasma levels of lysophosphatidic acid (LPA) and other lysophospholipids. We measured activities of lysophospholipase D (lysoPLD) in plasma and lipid phosphate phosphatase (LPP) in blood by determining choline and inorganic phosphate, respectively. LPA, lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI), lysophosphatidylserine (LPS) and lysophosphatodylglycerol were quantified by LC-MS/MS. In adrenalectomized rats, plasma levels of LPA, LPE, LPS and LPI, but not LPC, were increased. The increased level of LPA were due to decreased LPC level, increases plasma activity of lysoPLD toward LPC and decreased LPP activity toward LPA. Daily injections of deoxycoricosterone into rats selectively reversed increased level of LPS. Our results suggest enzymatic mechanism for increased plasma level of LPA, and indicate that the circulating levels of lysophospholipids including LPA in rats are differently affected by artificial suppression of release of adrenergic hormones.

Keywords: Deoxycorticosterone; Lipid phosphate phosphatase; Liquid chromatography-tandem mass spectrometry; Lysophosphatidic acid; Lysophosphatidylcholine; Lysophospholipase; Lysophospholipase D; Rat plasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Lysophospholipids*

Substances

Lysophospholipids
lysophosphatidic acid