The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.
Keywords: Cholesterol; Lipid metabolism; Lipid/Cholesterol biodistribution; Oral fat tolerance; Organ uptake; Radioactive tracer study.
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