USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3

Baoshan Cai; Jian Zhao; Yuling Zhang; Yaxing Liu; Chunhong Ma; Fan Yi; Yi Zheng; Lei Zhang; Tian Chen; Huiqing Liu; Bingyu Liu; Chengjiang Gao

doi:10.1080/15548627.2021.1965426

USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3

Autophagy. 2022 May;18(5):990-1004. doi: 10.1080/15548627.2021.1965426. Epub 2021 Sep 5.

Authors

Baoshan Cai¹, Jian Zhao¹, Yuling Zhang¹, Yaxing Liu¹, Chunhong Ma¹, Fan Yi², Yi Zheng¹, Lei Zhang¹, Tian Chen³, Huiqing Liu², Bingyu Liu¹, Chengjiang Gao¹

Affiliations

¹ Key Laboratory of Infection and Immunity of Shandong Province & Department of Immunology, School of Biomedical Sciences, Shandong University, Jinan, China.
² Department of Pharmacology, School of Biomedical Sciences, Shandong University, Jinan, China.
³ Department of Pathogenic Biology, School of Biomedical Sciences, Shandong University, Jinan, China.

Abstract

3-MA: 3-methyladenine; AIM2: absent in melanoma 2; ATG5: autophagy related 5; BafA1: bafilomycin A₁; CASP1: caspase 1; CHX: cycloheximide; Co-IP: co-immunoprecipitation; CQ: chloroquine; DUBs: deubiquitinases; IL1B/IL-1β: interleukin 1 beta; LAMP1: lysosomal associated membrane protein 1; LPS: lipopolysaccharide; MARCHF7/MARCH7: membrane associated RING-CH-type finger 7; NFKB/NF-κB: nuclear factor kappa B; Nig.: nigericin; NLRC4: NLR family CARD domain containing 4; NLRP3: NLR family pyrin domain containing 3; PECs: peritoneal exudate cells; PMN: polymorphonuclear; PMs: peritoneal macrophages; PYCARD/ASC: PYD and CARD domain containing; TLRs: toll like receptors; TNF/TNF-α: tumor necrosis factor; Ub: ubiquitin; USP5: ubiquitin specific peptidase 5; WT: wild type.

Keywords: Autophagy-lysosome pathway; MARCHF7; NLRP3 inflammasome; USP5; deubiquitinase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy
Caspase 1 / metabolism
Inflammasomes* / metabolism
Interleukin-1beta / metabolism
Lipopolysaccharides / pharmacology
NF-kappa B / metabolism
NLR Family, Pyrin Domain-Containing 3 Protein* / metabolism

Substances

Inflammasomes
Interleukin-1beta
Lipopolysaccharides
NF-kappa B
NLR Family, Pyrin Domain-Containing 3 Protein
Caspase 1

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China [31730026, 81930039, 81525012 to C.G., 31900680 to B.L. and 82001678 to T.C.]. This work was also supported by the National Postdoctoral Program for Innovative Talents [BX201700146 to B.L.] and Shandong Provincial Natural Science Foundation [ZR2018BC021 to B.L.].